Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Mus musculus | |
dithiothreitol | - |
Homo sapiens | |
dithiothreitol | - |
Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ngamma-monomethyl-L-arginine | endothelial and neuronal isoforms: reversible inhibition; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site | Homo sapiens | |
Ngamma-monomethyl-L-arginine | inducible isoform: after preincubation irreversible, time- and concentration-dependent inactivation, without preincubation reversible inhibition; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site | Mus musculus | |
Ngamma-monomethyl-L-arginine | endothelial and neuronal isoforms: reversible inhibition; in presence of tetrahydrobiopterin 0.004 mM the neuronal isoform is inactivated; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site | Rattus norvegicus | |
Ngamma-nitro-L-arginine | irreversible inactivation of neuronal and endothelial isoform after preincubation, unaffected by tetrahydrobiopterin; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site | Homo sapiens | |
Ngamma-nitro-L-arginine | L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site; reversible inhibitor of inducible isoform from macrophage | Mus musculus | |
Ngamma-nitro-L-arginine | irreversible inactivation of neuronal and endothelial isoform after preincubation, unaffected by tetrahydrobiopterin; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Mus musculus | |
Ca2+ | - |
Homo sapiens | |
Ca2+ | - |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
Sprague-Dawley | - |
Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
brain | - |
Homo sapiens | - |
brain | - |
Rattus norvegicus | - |
cerebellum | - |
Homo sapiens | - |
cerebellum | - |
Rattus norvegicus | - |
endothelium | - |
Mus musculus | - |
endothelium | - |
Rattus norvegicus | - |
endothelium | umbilical vein cells | Homo sapiens | - |
macrophage | - |
Homo sapiens | - |
macrophage | - |
Rattus norvegicus | - |
macrophage | RAW 264.7 cells | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Homo sapiens | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus Sprague-Dawley | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
e-NOS | endothelial isoform III | Homo sapiens |
i-NOS | isoform II, inducible | Mus musculus |
i-NOS | isoform II, inducible | Homo sapiens |
i-NOS | isoform II, inducible | Rattus norvegicus |
n-NOS | isoform I, neuronal enzyme | Homo sapiens |
n-NOS | isoform I, neuronal enzyme | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
7.4 | - |
assay at | Homo sapiens |
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydro-L-biopterin | - |
Mus musculus | |
5,6,7,8-tetrahydro-L-biopterin | - |
Homo sapiens | |
5,6,7,8-tetrahydro-L-biopterin | - |
Rattus norvegicus | |
Calmodulin | - |
Mus musculus | |
Calmodulin | - |
Homo sapiens | |
Calmodulin | - |
Rattus norvegicus | |
NADPH | - |
Mus musculus | |
NADPH | - |
Homo sapiens | |
NADPH | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
Ngamma-nitro-L-arginine | irreversible inactivation of endothelial isoform after preincubation | Homo sapiens | |
0.00009 | - |
Ngamma-nitro-L-arginine | irreversible inactivation of neuronal isoform after preincubation | Rattus norvegicus | |
0.0007 | - |
Ngamma-monomethyl-L-arginine | - |
Rattus norvegicus | |
0.0007 | - |
Ngamma-monomethyl-L-arginine | endothelial isoform | Homo sapiens | |
0.002 | - |
Ngamma-monomethyl-L-arginine | neuronal isoform in presence of 0.004 mM tetrahydrobiopterin | Rattus norvegicus | |
0.0026 | - |
Ngamma-monomethyl-L-arginine | inducible isoform from macrophage after preincubation | Mus musculus | |
0.0039 | - |
Ngamma-monomethyl-L-arginine | inducible isoform from macrophage without preincubation | Mus musculus | |
0.0065 | - |
Ngamma-monomethyl-L-arginine | neuronal isoform | Rattus norvegicus | |
0.0081 | - |
Ngamma-nitro-L-arginine | reversible inhibition of inducible macrophage isoform | Mus musculus |